Mariano Buffone, PhD
$150,000 / 2 years Awarded November 2020 |
Mariano Buffone, PhDInstituto de Biologia y Medicina Experimental (IBYME-CONICET)
Buenos Aires, Argentina About
Dr. Buffone is a Scientific Researcher from the National Research Council (CONICET) at Argentina’s Instituto de Biologia y Medicina Experimental (IBYME-CONICET) in Buenos Aires. Mariano has been working in the field of sperm physiology for two decades. His laboratory seeks to understand the complex process of mammalian sperm capacitation. To this end, his lab uses a wide range of approaches that include the analysis of signaling pathways, single cell imaging experiments, super resolution microscopy and in vivo studies. Dr Buffone has also been very active collaborating with several groups in the United States, Japan, Mexico and Argentina. Our Grant
MCI is supporting the Buffone lab and their studies on the sperm-specific calcium channel CatSper. Their proposal seeks to use novel high-throughput methods to screen drugs with the capacity to block CatSper. New compounds that inhibit this channel in this initial phase will be refined in a second phase to improve selectivity, potency and adequate drug like properties for in vivo preclinical studies. |
MCI Grantee: Instituto de Biologia y Medicina Experimental (Dr. Mariano Buffone)
Publications
Luque Guillermina M., Schiavi-Ehrenhaus Liza J., Jabloñski Martina, Balestrini Paula A., Novero Analia G., Torres Nicolás I., Osycka-Salut Claudia E., Darszon Alberto, Krapf Dario, Buffone Mariano G. 'High-throughput screening method for discovering CatSper inhibitors using membrane depolarization caused by external calcium chelation and fluorescent cell barcoding', Frontiers in Cell and Developmental Biology, 11 (2023)
Stival, Cintia, Lis del C. Puga Molina, Bidur Paudel, Mariano G. Buffone, Pablo E. Visconti, and Dario Krapf, ‘Sperm Capacitation and Acrosome Reaction in Mammalian Sperm’, Advances in Anatomy, Embryology, and Cell Biology, 220 (2016), 93–106
Puga Molina, Lis C., Guillermina M. Luque, Paula A. Balestrini, Clara I. Marín-Briggiler, Ana Romarowski, and Mariano G. Buffone, ‘Molecular Basis of Human Sperm Capacitation’, Frontiers in Cell and Developmental Biology, 6 (2018), 72
Luque Guillermina M., Schiavi-Ehrenhaus Liza J., Jabloñski Martina, Balestrini Paula A., Novero Analia G., Torres Nicolás I., Osycka-Salut Claudia E., Darszon Alberto, Krapf Dario, Buffone Mariano G. 'High-throughput screening method for discovering CatSper inhibitors using membrane depolarization caused by external calcium chelation and fluorescent cell barcoding', Frontiers in Cell and Developmental Biology, 11 (2023)
Stival, Cintia, Lis del C. Puga Molina, Bidur Paudel, Mariano G. Buffone, Pablo E. Visconti, and Dario Krapf, ‘Sperm Capacitation and Acrosome Reaction in Mammalian Sperm’, Advances in Anatomy, Embryology, and Cell Biology, 220 (2016), 93–106
Puga Molina, Lis C., Guillermina M. Luque, Paula A. Balestrini, Clara I. Marín-Briggiler, Ana Romarowski, and Mariano G. Buffone, ‘Molecular Basis of Human Sperm Capacitation’, Frontiers in Cell and Developmental Biology, 6 (2018), 72
